Wheat-germ agglutination of Neisseria gonorrhoeae. A laboratory investigation.
نویسندگان
چکیده
Wheat-germ agglutination (WGA) was used to identify 168 strains of Neisseria gonorrhoeae and 105 strains of other Neisseria species in a routine laboratory. About one-third of the meningococci reacted with the lectin and titres with some organisms varied on repeat testing. The technique is regarded as unreliable for the identification of Neisseria species.
منابع مشابه
Lectins in diagnostic microbiology: use of wheat germ agglutinin for laboratory identification of Neisseria gonorrhoeae.
A lectin slide agglutination test has been developed for the confirmatory identification of Neisseria gonorrhoeae. With wheat germ lectin as an agglutinin, 164 of 165 clinical isolates of N. gonorrhoeae gave a 3 to 4+ reaction within 6 to 8 min. Four gonococcal isolates, even though negative by the fluoresecent-antibody method, gave strong positive reactions with the wheat germ lectin. Among 23...
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The lectin slide agglutination test for Neisseria gonorrhoeae has been modified and improved. Results show that wheat germ agglutinin and soybean lectin agglutinate 100% (193 of 193 tested) of clinical isolates of N. gonorrhoeae. Lectin-reactive meningococci can be readily identified by the hydrolysis of gammaglutamyl-3-naphthylamide. Branhamella catarrhalis, Neisseria lactamica, Neisseria sicc...
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A group of five tests utilizing wheat germ and soybean lectins and chromogenic substrates (orthonitrophenyl-beta-D-galactopyranoside, gamma-glutamyl-beta-naphthylamide, and prolyl-beta-naphthylamide derivatives) was used as a rapid (30-min) method for the identification of Neisseria gonorrhoeae. The rapid method agreed with Minitek test results for all 126 N. gonorrhoeae isolates and all 39 non...
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Antiserum to a purified type R lipopolysaccharide antigen isolated from Neisseria gonorrhoeae was used in a slide agglutination test and compared with conventional carbohydrate utilization and fluorescent antibody tests to confirm the identity of laboratory cultures classified as typical or "atypical" N. gonorrhoeae. Cultures of Corynebacterium vaginalis, N. meningitidis, N. catarrhalis, N. sic...
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ورودعنوان ژورنال:
- The British journal of venereal diseases
دوره 57 4 شماره
صفحات -
تاریخ انتشار 1981